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Descripción
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| 18 The bZIP transcription factor AtbZIP44 during seed germination: Regulation of the mannanase encoding gene AtMAN7. Raquel Iglesias-Fernández1, Cristina Barrero-Sicilia1, Néstor Carrillo-Barral2, Luis Oñate-Sánchez1, Pilar Carbonero1 1Centro de Biotecnología y Genómica de Plantas (UPM-INIA), ETSI Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid (Spain) 2Departamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782, Santiago de Compostela (Spain) email: raquel.iglesias@upm.es During Arabidopsis thaliana seed germination, the weakening of the endosperm cell walls (CWs) is a key step needed to reach germination sensu stricto. Dismantling of these CWs is driven by hydrolytic enzymes such are endo-?-mannanases (MAN; EC. 3.2.1.78) that catalyze the cleavage of the ?1?4 bonds in the mannan backbone; this activity increases upon seed imbibition. Transcripts of the most highly expressed AtMAN gene during seed germination (AtMAN7) are restricted to the micropylar endosperm and to the radicle tip just before radicle emergence. To gain insight into the cis-, trans- transcriptional regulation of the AtMAN7 gene, a phylogenetic shadowing process has been pursued to find conserved cis-elements in the promoters of the Brassicaceae orthologous MAN7 genes and these conserved motives have been used as baits in a 1-hybrid screening, using as a prey, an arrayed yeast library of circa 1,200 Transcriptional Factor Open Reading Frames (TF ORFs) from A. thaliana. The basic-leucine zipper AtbZIP44, has been thus identified and its regulatory function upon AtMAN7 during seed germination validated by different molecular and physiological techniques and by the establishment of the germination kinetics of both over-expression (oex) and T-DNA insertion mutant lines of AtbZIP44. The transcriptional combinatorial network through which AtbZIP44 regulates AtMAN7 gene expression during seed germination has been further explored through protein-protein interactions between AtbZIP44 and other TFs. | |
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Internacional
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Si |
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Nombre congreso
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4th International Society for Seed Science (ISSS) Workshop on Molecular Aspects of Seed Dormancy and Germination. |
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Tipo de participación
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960 |
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Lugar del congreso
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Paris (Francia) |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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09/07/2013 |
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Fecha fin congreso
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12/07/2013 |
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Desde la página
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18 |
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Hasta la página
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18 |
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Título de las actas
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4th International Society for Seed Science (ISSS) Workshop on Molecular Aspects of Seed Dormancy and Germination. |