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Memorias de investigación
Communications at congresses:
Cold signalling and circadian clock gene expression converge at the promoter level in poplar
Year:2014
Research Areas
  • Agriculture
Information
Abstract
Trees from temperate regions have developed an adaptive mechanism called winter dormancy aimed to survive the extreme climate changes that take place during this season. The circadian clock is involved in the transduction of daylenght and temperature signals that regulate the winter dormancy. In chestnut in response to cold, circadian clock genes lose their rhythmic expression and become constitutively activated (1, 2). This molecular response to cold also occurs in Arabidopsis but only when light/dark cues are absent (3). We are interested in understanding how this specific molecular response is controlled in order to shed light on the biological meaning of the cold-induced constitutive activation of circadian clock genes in perennials. Our hypothesis is that cold-induceddisruption of circadian clock genes in woody plants is due to a transcriptional control operating above these genes. First of all we wanted to check whether poplar, the model tree specie, showed this alteration as well as chestnut. Once we had confirmed the disruption in natural conditions, we cloned the promoter of a circadian clock gene from Populus tremula x alba fused to the luciferase reporter. Poplar plants stably transformed with this construct were used to perform luminescence assays under different photoperiods and temperature conditions. Our results indicate that the selected promoter fragment contains the elements both to keep the circadian rhythm at 21ºC and to drive the cold-induced disruption of circadian clock gene expression.
International
No
Congress
XII Reunión de Biología Molecular de Plantas
960
Place
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
11/06/2014
End Date
13/06/2014
From page
232
To page
232
XII Reunión de Biología Molecular de Plantas
Participants
  • Autor: Jose Manuel Ramos Sanchez (UPM)
  • Autor: Tamara Hernández-Verdeja (UPM)
  • Autor: Alicia Moreno Cortes (UPM)
  • Autor: Isabel Marta Allona Alberich (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)