Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Comparative genomics of lupin rhizobial symbionts
Year:2015
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Native bradyrhizobial symbionts of lupins thriving in the Iberian Peninsula were studied. The analysis included five species that preferred neutral or acid soils: L. angustifolius, L. luteus, L. hispanicus, L. micranthus and L. cosentinii, and the unique, recently described lupin species L. mariae-josephae, endemic of alkaline Chromic Luvisol soils (?terra rossa?) in Eastern Spain. Draft genome sequences of at least one strain from each lupin species were obtained by Illumina (PE, 2x300 bp, MySeq v. 3.0) sequencing, assembled with SPAdes, and annotated with the RAST server. Pairwise Average Nucleotide Identities (ANI) between genomes were calculated using JSpecies. The results showed a clear separation between strains nodulating L. mariae-josephae and those that nodulate lupins from acid soils. These results are in agreement with the observed host specificity, i. e. bradyrhizobia from L. mariae-josephae don?t nodulate most of the lupins from acid soils and any bradyrhizobia from acid soil lupins do nodulate, although inefficiently, L. mariae-josephae. In contrast, acid soil lupins are efficiently nodulated by bradyrhizobia isolated from any of these lupins. Consistent with this, comparison of symbiotic regions (nod, fix, nif genes), showed maximum differences between L. mariae-josephae strains and those from other lupins. Another genomic features were also compared and results will be discussed.
International
Si
Congress
14th International Lupin Conference
960
Place
Milán (Italia)
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
21/06/2015
End Date
26/06/2015
From page
144
To page
144
14th International Lupin Conference
Participants
  • Autor: Luis Rey Navarro (UPM)
  • Autor: David Ricardo Duran Wendt (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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