Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Molecular determinats for the intercellular diffusion barrier between oligodendrocytes and astrocyte
Year:2015
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Direct intercellular communication via gap junction channels among oligodendrotrocytes and astrocytes themselves and between each other is essential to maintain the functionality and integrity of central myelin. In that regards, oligodendrocyte gap junction connexin-47 (Cx47) constitutes a key element since Cx47 mutations in humans cause severe defects of myelination and demyelination. Cx47 is the principal component of intercellular channels that account for the weak coupling among oligodentrocytes and, by forming heterotypic channels with astrocyte connexin-43 (Cx43), of heterologous oligodendrocyte-astrocyte junctions that act as a diffusion barrier to the movement of molecules between oligodentrocytes and astrocytes. The determinants of pore channel structure responsible for the permeability characteristics of coupling interoligodendrocytes and that generate ?oligodentrocyte-astrocyte rectifier? were investigated. We found that the restrictive permeability of Cx47 channels are due to the presence of three extra residues (Met-Thr-Asn) at 5¿ extreme of aminoterminal domain of full-length Cx47 and, therefore, to the strong electrostatic interaction of Met1 with the pore exposed residue Glu44 located at the narrower constriction site of pore tunnel. In turn, the asymmetrical ionic rectification with the transjunctional voltage polarity of Cx47-Cx43 channels and the unidirectional passage of negatively but not of positively, charged dyes from Cx47 side to Cx43 cell are generated on the basis of the more restrictive permeability of Cx47 hemichannel. Interestingly, a disease-related mutation of Cx47, the P90S, abolished the restrictive properties of permeability of Cx47 channels and the directionality of Cx47-Cx43 channels, suggesting that they may be of physiological relevance.
International
Si
Congress
5th Spanish Ion Channel Network Meeting (RECI): Present and Future Research in Ion Channel Research
970
Place
BARCELONA
Reviewers
Si
ISBN/ISSN
0000-0000-00
Start Date
04/10/2015
End Date
06/10/2015
From page
60
To page
60
ABSTRACTS BOOK RECI
Participants
  • Autor: Daniel Gonzalez Nieto (UPM)
  • Autor: LC BARRIO
  • Autor: P MARTINEZ MONTERO
  • Autor: J MOLANO
  • Autor: I FASCIANI
  • Autor: P PLUTA
  • Autor: O MILLET
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Tecnologías para Ciencias de la Salud
  • Departamento: Tecnología Fotónica y Bioingeniería
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)