Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Assessment of genetic drift in ex situ conserved seeds of rocket (Eruca vesicaria) after regeneration
Year:2016
Research Areas
  • Plants and animal biology and ecology
Information
Abstract
Four accessions of rocket [Eruca vesicaria (L.) Cav.] of wild origin held at the Universidad Politécnica de Madrid germplasm bank were regenerated in isolated cages with bumblebees as pollinators. The genetic diversity of parental (P) and regenerated (R) populations was studied by means of molecular markers: RAPD and two Brassica rapa SSR loci. From RAPD markers, UPGMA cluster analysis was performed; diversity was estimated using Shannon?s index and AMOVA. Differences in marker frequency between P and R populations of each accession were analysed through the c2 test. Frequencies of SSR alleles, genetic diversity estimators (FST, FIS) and AMOVA were calculated. The results obtained with RAPD markers were in accordance with those from SSRs and indicate that the regeneration process had only minor effects on the genetic diversity of samples in three out of four studied accessions. Detected changes in the frequency of markers were in most cases negligible and do not seem to constitute either a significant loss of genetic variability or risk of genetic erosion, except when seed dormancy or other factors involving selection are acting, as it might have happened in Ev9107. It has been demonstrated that Brassica rapa SSR markers can be effectively transferred to Eruca vesicaria.
International
Si
JCR
Si
Title
Seed Science And Technology
ISBN
0251-0952
Impact factor JCR
0,706
Impact info
Datos JCR del año 2013
Volume
44
Journal number
From page
1
To page
15
Month
SIN MES
Ranking
Participants
  • Autor: Juan Bautista Martinez Laborde (UPM)
  • Autor: Miguel Angel Ibañez Ruiz (UPM)
  • Autor: Elena Rey-Mazón
  • Autor: Natalia Pita-Torres
  • Autor: David Draper
  • Autor: Maria Carmen Martin Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Economía Agraria, Estadística y Gestión de Empresas
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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