Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
DNA demethylases control growth-dormancy transitions in Poplar
Year:2016
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Coordinating growth and reproduction with the environment is essential for the survival of trees in temperate and boreal latitudes. In these regions, deciduous and periodic growth habits evolved into a single trait known as winter dormancy. This trait consists of growth cessation through the arrest of meristem activity and consequent transformation of the apical meristem into a dormant winter bud. For many trees and perennial plants, the perception of short days (SD) is sufficient to promote cessation of growth and bud formation. Restoration of meristem activity and bud break during the spring are trigged by the environmental favourable conditions (long days and warm temperatures) once the chilling requirement is fulfilled. Active chromatin rearrangement has been proposed as key steps in growth-dormancy transitions in trees, but yet functional studies are needed to support this hypothesis. In this work we investigated the role of DNA demethylases during dormancy. Thought phylogenetic and protein sequence analyses we firstly identified poplar and chestnut Demeter-like genes (DMLs). Expression studies showed that CsDML and its closer homolog gene PtaDML6, are induced during dormancy entrance. Overexpression of CsDML accelerated short day-induced bud formation in poplar. Comparative transcriptional profiling revealed that the overexpression of this 5mC DNA demethylase promoted the molecular changes previously observed under short-day conditions. In the other hand, two poplar DMLs genes, PtaDML8 and PtaDML10, were induced during dormancy exit. The phenological assays showed that PtaDML8-10 knockdown plants (KD) have a delayed bud break. Transcriptome analyses revealed that KD plants have altered biological processes such as cellular metabolic process, photosynthesis, ribosome biogenesis and response to light and temperature stimulus. Whole Genome Bisulphite Sequencing let us to identify the DMR (differentially methylated region) in KDs vs. WT at bud break, indicating that transcriptomic changes could be associated to the changes in DNA methylation status. Taken all together, we demonstrate for the first time that chromatin rearrangement leading by active DNA demethylation control dormancy entrance and exit in poplar.
International
No
Congress
XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
960
Place
Oviedo
Reviewers
Si
ISBN/ISSN
CDP08UPM
Start Date
22/06/2016
End Date
24/06/2016
From page
80
To page
80
XIII REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
Participants
  • Autor: Daniel Conde Rodriguez (UPM)
  • Autor: Alicia Moreno Cortes (UPM)
  • Autor: Anne-Laure Le Gac
  • Autor: Christopher Dervinis
  • Autor: Jose Manuel Ramos Sanchez (UPM)
  • Autor: Matias Kirst
  • Autor: Stéphane Maury
  • Autor: Mariano Manuel Perales (UPM)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Isabel Marta Allona Alberich (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Grupo de Investigación: Biotecnología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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