Observatorio de I+D+i UPM

Memorias de investigación
Expression of Nitrogenase Component Proteins is Eukaryotes. The nitrogen nutrition of plants.
Research Areas
  • Natural sciences and health sciences
The extensive use of synthetic N fertilizers in developed countries poses enormous environmental threats that must be addressed. The awareness that biological N2 fixation can be used as alternative to the synthetic N fertilizers in the implementation of modern sustainable agricultural practices is an underlying driving force for nitrogenase studies. An ambitious challenge of plant biotechnology is to increase cereal crop productivity by engineering plants to fix their own nitrogen, i.e. by functional expression of bacterial N2 fixation (nif) genes in the plant. Two identified barriers have traditionally impaired this approach: the known sensitivity of nitrogenase to O2 (the by-product of plant photosynthesis) and the genetic/biochemical complexity of nitrogenase biosynthesis. Balanced expression of at least 9 nif gene is required to mature nitrogenase structural polypeptides into their catalytically active forms. Both nitrogenase components, as well as most proteins required for the assembly of their metal clusters, are very sensitive to O2. Here we present data demonstrating that the mitochondrial matrix provides a low O2 environment appropriate for the assembly and the activity of nitrogenase components. In addition, a combined synthetic biology and biochemical complementation approach is being used to tackle the problem of nitrogenase genetic complexity. Advances towards expressing a full nitrogenase complex in eukaryotic cells will be presented.
EMBO Conference: The nitrogen nutrition of plants
Entity Nationality
Sin nacionalidad
  • Autor: Gema Lopez Torrejon (UPM)
  • Autor: Elena Caro Bernat (UPM)
  • Autor: Nils Stefan Buren (UPM)
  • Autor: Carlos Echavarri Erasun (UPM)
  • Autor: Emilio Jimenez Vicente (UPM)
  • Autor: José María Buesa Galiano (UPM)
  • Autor: Mónica Navarro Rodríguez (UPM)
  • Autor: Ana Perez Gonzalez (UPM)
  • Autor: Marcel Veldhuizen . (UPM)
  • Autor: Luis Manuel Rubio Herrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)