Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
MtNramp1, MtZIP6, and MtCOPT1 are Respectively Responsible for Iron, Zinc, and Copper Uptake by Medicago truncatula Nodule Cells
Year:2016
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Legume nodules require relatively large amounts of transition metals (iron, copper, zinc?) as cofactors of some of the key enzymes involved in symbiotic nitrogen fixation (1). These metals are provided by the host plant through the vasculature and, in the case of indeterminate type nodules, released in the infection/differentiation zone of the nodule (2). Consequently, a number of metal transporters must be in place to introduce these elements into the nodule cells to be later delivered to the symbiosomes. We have identified three Medicago truncatula metal transporters encoding genes, MtNramp1 (3), MtZIP6, and MtCOPT1, that are expressed at high levels in the nodule zone where metals have to be incorporated from the apoplast. Yeast complementation assays show that they are respectively involved in iron, zinc, and copper transport towards the cytosol. Consistent with a role in metal uptake from the apoplast, they are located in the plasma membrane of nodule cells. Knock out or knock-down plants of each gene have reduced growth and reduced nitrogenase activity. These phenotypes were restored by reintroducing a wild type form of the mutated gene or by watering the plants with a nutritive solution fortified in the corresponding transported metal. These results are consistent with a model in which MtNramp1 is responsible for Fe2+ loading of nodule cells, while MtZIP6 is for Zn2+ uptake, and MtCOPT1 for Cu+.
International
Si
Congress
12th European Nitrogen Fixation Conference
960
Place
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
25/08/2016
End Date
28/08/2016
From page
123
To page
123
12th European Nitrogen Fixation Conference
Participants
  • Autor: Manuel Tejada Jiménez (UPM)
  • Autor: Angela Enriqueta Saez Somolinos (UPM)
  • Autor: Marta Senovilla Ramos (UPM)
  • Autor: M del Rosario Castro Rodriguez (UPM)
  • Autor: Isidro Abreu (UPM)
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: Igor Kryvoruchko (Noble Foundation)
  • Autor: M. Mercedes Lucas (CSIC)
  • Autor: Michael Udvardi (Noble Foundation)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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