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Descripción
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| Bdellovibrio bacteriovorus is an obligate predatory bacterium that preys upon a wide range of Gram negative bacteria, including polyhydroxyalkanoates (PHA) producing bacteria such as Pseudomonas putida. The biphasic growth cycle of Bdellovibrio includes a free-swimming attack phase and an intraperiplasmic growth phase, where Bdellovibrio replicates its DNA and grows using the prey as a source of nutrients. The PHA belongs to a biodegradable and biocompatible polymeric family synthesized by a wide variety of microorganisms during adverse nutritionals conditions. This biopolymer has been broadly studied due to applications in pharmacology and medical technology because its physico-chemical properties and has been postulated as an excellent alternative to the petroleum-based derivate materials. Previous work from our lab demonstrated that PHA accumulation doesn¿t protect P. putida from B. bacteriovorus predation and that this predator can degrade the biopolymer using a specific extracellular mcl-PHA depolymerase (PhaZBd). In this work, the predation by B. bacteriovorus on different strains of P. putida has been characterized. To this aim, the influence of the viable cell number of Bdellovibrio at the start of the experiment has been studied. The progeny and the cell biomass have been analyzed, suggesting that the initial population of the predator has a linear relation with the prey¿s reduction, while the cell biomass increases after predation. The quantification of the intracellular ATP content reveals that the PHA has a great impact in the energetic state of Bdellovibrio, because the polymer can be use as extra energy source. However, any evidence of changes in predator progeny or predation process has not been detected. | |
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Internacional
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Tipo de Tesis
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Doctoral |
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Calificación
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Sobresaliente |
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