Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
BioPhotonics Sensing Cells for in-situ dry eye screening
Year:2017
Research Areas
  • Biosensors
Information
Abstract
An integrated optical biosensor is used for label-free detection and a competitive limit of detection is obtained. The purpose sensor is fabricated by contact lithography technique [1] and a bio-compatible multilayer interferometric transducer is created. The resulting biosurface makes the immobilization of any possible bioreceptor easier and allows a vertical optical characterization of the biosensor. In this case a label-free detection of protein S100A6 related with dry eye disease is performed. To accomplish the S100A6 detection, in first place only 3 µL of protein A is incubated. Through this linker, a desired orientation of the antibody (bioreceptor) can lead to a better recognition. After protein A , 3 µL of 20 µg/mL antiS100A6 solution is incubated. The biofunctionalization process finishes with the incubation of 3% BSA solution as blocking agent to avoid unspecific recognition. Finally, the detection of the S100A6 is performed using different concentrations solutions of 3 µL. As a result, the recognition curve shows that the proposed biosensor is capable to detect the protein in a detection range from ng/mL to µg/mL with very low sample volume.
International
Si
Congress
II Workshop in Biomaterial an Applications
970
Place
Santiago de Compostela
Reviewers
No
ISBN/ISSN
000-00-00000-00-0
Start Date
27/04/2017
End Date
28/04/2017
From page
51
To page
51
BioPhotonics Sensing Cells for in-situ dry eye screening II Workshop in Biomaterial an Applications
Participants
  • Autor: Beatriz Santamaria Fernandez (UPM)
  • Autor: Maria Fe Laguna Heras (UPM)
  • Autor: David López-Romero (BioD)
  • Autor: Ana Lopez Hernandez (UPM)
  • Autor: Francisco Javier Sanza Gutierrez (UPM)
  • Autor: Alvaro Lavin Hueros (UPM)
  • Autor: Rafael Casquel Del Campo (UPM)
  • Autor: Mª Victoria Maigler López (UPM)
  • Autor: Rocio Lopez Espinosa (UPM)
  • Autor: Miguel Holgado Bolaños (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Grupo de Óptica Fótonica y Biofotónica (GOFB)
  • Centro o Instituto I+D+i: Centro Laser
  • Departamento: Física Aplicada e Ingeniería de Materiales
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Tecnología Fotónica y Bioingeniería
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)