Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
MEDICAGO TRUNCATULA FERROPORTIN2 (MTFPN2) IS A NODULE- SPECIFIC IRON EXPORTER
Year:2017
Research Areas
  • Molecular, cellular and genetic biology,
  • Microbiology
Information
Abstract
Legumes are able to use atmospheric nitrogen and convert it to ammonia through a partnership with endosymbiotic rhizobia. This symbiotic nitrogen fixation (SNF) occurs in root nodules formed with rhizobia. SNF requires different transition metals, notably iron, as essential cofactors of multiple proteins involved in this process (leghemoglobin, nitrogenase?). Previous studies in our laboratory showed that iron is driven from vascular bundles to the aploplast of the infection zone of the nodule (1). Transcriptomic studies in Medicago truncatula nodules showed that MtFPN2 is specific to the nodule. Within this organ, promoter::gus fusions and immunolocalization showed that it was located in the nodule vasculature and in some intracellular compartment in rhizobia-infected cells. Yeast complementation assays in several metal transporter mutants, indicated that MtFPN2 was able to transport iron and manganese out of the cytosol, either into an organelle or across the plasma membrane. Phenotypical characterization of two Tnt1-insertion mutant alleles (fpn2-1 and fpn2-2) revealed that MtFPN2 was important for SNF, as both lines presented a significantly lower nitrogenase activity than wild type plants.
International
No
Congress
Reunión de la Sociedad Española de Fisiología Vegetal
970
Place
Reviewers
Si
ISBN/ISSN
0000000000
Start Date
26/06/2017
End Date
29/06/2017
From page
214
To page
214
Reunión de la Sociedad Española de Fisiología Vegetal
Participants
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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