Observatorio de I+D+i UPM

Memorias de investigación
Other publications:
'Expression of mitochondria targeted NifH in Nicotiana benthamiana'
Year:2018
Research Areas
  • Genetically modified organisms
Information
Abstract
Póster en la European Nitrogen Fixation Conference 2018. Abstract del póster: "The generation of cereal plants that express a functional nitrogenase, and thus utilizing atmospheric nitrogen, could revolutionize agricultural systems worldwide1,2. Two of the barriers that need to be addressed to achieve this goal are the functional expression of the large number of genetic parts required for the biochemically complex nitrogenase protein, and the sensitivity of their protein products towards O2. Targeting of nitrogenase components to the mitochondria could benefit from the presence of bacterial-type iron-sulfur cluster biosynthetic machinery and the organelle?s high O2 consumption3,4. One key step in nitrogenase biogenesis is the biosynthesis of NifB-co, the metabolic product of NifB5. NifB-co is a unique [Fe8-S9-C] cluster that serves as biosynthetic precursor to the iron- molybdenum cofactor (FeMo-co) of nitrogenase6. Because of its oxygen sensitivity, instability and complex metal requirements, obtaining in vivo NifB activity and NifB-co production is hypothesized to be one of the most difficult steps to engineer eukaryotic nitrogenase. Here, we will report results of expressing and purifying NifB from mitochondria of Saccharomyces cerevisiae, and from mitochondria and chloroplasts of Nicotiana benthamiana."
International
Si
Entity
European Nitrogen Fixation Conference 2018
Place
Estocolmo
Pages
Reference/URL
Publication type
Poster
Participants
  • Autor: XI Jiang (UPM)
  • Autor: Nils Stefan Buren (UPM)
  • Autor: Luis Manuel Rubio Herrero (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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