Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
MtMOT1.2 is responsible for molybdate supply to Medicago truncatula nodules
Year:2018
Research Areas
  • Molecular biology,
  • Plant genetics
Information
Abstract
Symbiotic nitrogen fixation in legume root nodules requires a steady supply of molybdenum for synthesis of the iron?molybdenum cofactor of nitrogenase. This nutrient has to be provided by the host plant from the soil, crossing several symplastically disconnected compartments through molybdate transporters, including members of the MOT1 family. Medicago truncatula Molybdate Transporter (MtMOT) 1.2 is a Medicago truncatula MOT1 family member located in the endodermal cells in roots and nodules. Immunolocalization of a tagged MtMOT1.2 indicates that it is associated to the plasma membrane and to intracellular membrane systems, where it would be transporting molybdate towards the cytosol, as indicated in yeast transport assays. Loss?of?function mot1.2?1 mutant showed reduced growth compared with wild?type plants when nitrogen fixation was required but not when nitrogen was provided as nitrate. While no effect on molybdenum?dependent nitrate reductase activity was observed, nitrogenase activity was severely affected, explaining the observed difference of growth depending on nitrogen source. This phenotype was the result of molybdate not reaching the nitrogen?fixing nodules, since genetic complementation with a wild?type MtMOT1.2 gene or molybdate?fortification of the nutrient solution, both restored wild?type levels of growth and nitrogenase activity. These results support a model in which MtMOT1.2 would mediate molybdate delivery by the vasculature into the nodules.
International
Si
JCR
No
Title
Plant, Cell & Environment
ISBN
0140-7791
Impact factor JCR
Impact info
Volume
10.1111/pce.13388
Journal number
From page
1
To page
11
Month
JUNIO
Ranking
Participants
  • Autor: Patricia Gil Diez (UPM)
  • Autor: Manuel Tejada Jiménez (UPM)
  • Autor: Javier Leon Mediavilla (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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