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Memorias de investigación
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New roles of zf1-3 transcription factors in the control of nitrogen assimilation and export during vegetative growth
Year:2018
Research Areas
  • Biological sciences
Information
Abstract
Nitrogen is an essential macronutrient whose availability in soil is crucial for plant growth and crop productivity. Nitrate is the main source of inorganic nitrogen for land plants. However, it is usually limiting in the majority of the ecosystems, due to a variety of biotic and abiotic factors like microbial consumption, water leaching and soil erosion. Besides a macronutrient, nitrate is also a signal molecule that regulates the expression of a variety of genes that modulate many aspects of metabolism, growth and development. Here we identify the roles of ZF1 and ZF3, Arabidopsis DOF transcription factors in nitrate responses. Loss-of-function null mutant zf3-1 and zf1R exhibit nitrate-dependent root phenotypes, whereas ZF1 and ZF3 overexpression lines showed increased biomass and altered root architecture. Transcriptomic analyses of overexpressor lines indicated that both factors regulate the expression an important set of carbon and nitrogen regulated genes. Consistently, metabolite profiling disclosed that the total amount of key nitrogen metabolites including glutamate and glutamine were higher in ZF1/3-overexpressing plants in N-sufficient and limiting conditions. By contrast, zf3-1 and zf1R mutants exhibited lower amounts of those metabolites. In tomato, the overexpression of ZF1 and ZF3 genes modified amino acid and sugar content, improving biomass and yield. These results identify ZF1 and ZF3 as important regulatory factors of the nitrate response in Arabidopsis, suggesting that they are potential targets for improving nutrient use efficiency in crops.
International
Si
Congress
XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
970
Place
Salamanca
Reviewers
Si
ISBN/ISSN
0000-0000
Start Date
04/07/2018
End Date
06/07/2018
From page
168
To page
168
XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS
Participants
  • Autor: Jesus Vicente Carbajosa (UPM)
  • Autor: Joaquin Medina Alcazar (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)