Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
An experimental and modeling-based approach to locate IgE epitopes of plant profilin allergens.
Year:2007
Research Areas
  • Molecular biology
Information
Abstract
BACKGROUND: Plant profilins are actin-binding proteins that form a well-known panallergen family responsible for cross-sensitization between plant foods and pollens. Melon profilin, Cuc m 2, is the major allergen of this fruit. OBJECTIVE: We sought to map IgE epitopes on the 3-dimensional structure of Cuc m 2. METHODS: IgE binding to synthetic peptides spanning the full Cuc m 2 amino acid sequence was assayed by using a serum pool and individual sera from 10 patients with melon allergy with significant specific IgE levels to this allergen. Three-dimensional modeling and potential epitope location were based on analysis of both solvent exposure and electrostatic properties of the Cuc m 2 surface. RESULTS: Residues included in synthetic peptides that exerted the strongest IgE-binding capacity defined 2 major epitopes (E1, consisting of residues 66-75 and 81-93, and E2, consisting of residues 95-99 and 122-131) that partially overlapped with the actin-binding site of Cuc m 2. Two additional epitopes (E3, including residues 2-10, and E4, including residues 35-45) that should show weaker putative antigen-antibody associations and shared most residues with synthetic peptides with low IgE-binding capacity were predicted on theoretical grounds. CONCLUSIONS: Strong and weak IgE epitopes have been uncovered in melon profilin, Cuc m 2. CLINICAL IMPLICATIONS: The different types of IgE epitopes located in the 3-dimensional structure of melon profilin can constitute the molecular basis to explain the sensitization and cross-reactivity exhibited by this panallergen family.
International
No
JCR
No
Title
J Allergy Clin Immunol.
ISBN
17397911
Impact factor JCR
0
Impact info
Volume
119
Journal number
6
From page
1481
To page
1488
Month
JUNIO
Ranking
Participants
  • Autor: Gabriel Salcedo Duran (UPM)
  • Autor: Maria Rosa Sanchez-Monge Y Laguna de Rins (UPM)
  • Autor: Gema Lopez Torrejon (UPM)
  • Autor: Araceli Diaz Perales (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Departamento: Biotecnología
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