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Descripción
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| Electrophoresis has become one of the most usual tools for plant studies. However, most analyses, using isoenzymes, have been carried out from a population geneticist¿s point of view for the study of allelic frequencies. In present work, isoenzyme electrophoresis of bulked seeds was used as a rapid strategy for obtaining the electrophoretic profiles of accessions of a seed bank. The relationships amongst 30 taxa of the complex Diplotaxis-Erucastrum-Brassica (Brassicaceae family, tribe Brassicinae)were analysed, with the electrophoretic profiles of six isoenzyme systems. Each sample presented a stable and characteristic isoenzymatic pattern representative of the whole population. The enzyme markers provided 147 reliable bands which were scored as multistate traits (not with allelic value). UPGMA clustering method showed a dendrogram with three main groups and subgroups of samples that are in agreement with the recognised taxonomy of the taxa. This approach can be a rapid and efficient method to establish the specific enzyme patterns of conserved species populations of a germplasm bank, and the information could be included in the a database as part of documentation system. | |
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Internacional
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Si |
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JCR del ISI
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Si |
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Título de la revista
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GENETIC RESOURCES AND CROP EVOLUTION |
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ISSN
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0925-9864 |
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Factor de impacto JCR
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0,967 |
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Información de impacto
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Volumen
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56 |
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DOI
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10.1007/s10722-009-9423-5 |
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Número de revista
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7 |
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