Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Role of motility and chemotaxis in the pathogenesis of Dickeya dadantii 3937 (ex Erwinia chrysanthemi 3937)
Year:2009
Research Areas
  • Microbiology
Information
Abstract
Dickeya dadantii 3937 (ex Erwinia chrysanthemi), a member of the Enterobacteriaceae, causes soft rot in many economically important crops. A successful pathogen has to reach the interior of the plant in order to cause disease. To study the role of motility and chemotaxis in the pathogenicity of D. dadantii 3937, genes involved in the chemotactic signal transduction system (cheW, cheB, cheY and cheZ) and in the structure of the flagellar motor (motA) were mutagenized. All the mutant strains grew like the wild-type in culture media, and the production and secretion of pectolytic enzymes was not affected. As expected, the swimming ability of the mutant strains was reduced with respect to the wild-type: motA (94 %), cheY (80 %), cheW (74 %), cheB (54 %) and cheZ (48 %). The virulence of the mutant strains was analysed in chicory, Saintpaulia and potato. The mutant strains were also tested for their capability to enter into Arabidopsis leaves. All the mutants showed a significant decrease of virulence in certain hosts; however, the degree of virulence reduction varied depending on the virulence assay. The ability to penetrate Arabidopsis leaves was impaired in all the mutants, whereas the capacity to colonize potato tubers after artificial inoculation was affected in only two mutant strains. In general, the virulence of the mutants could be ranked as motA,cheY,cheB5cheW,cheZ, which correlated with the degree to which swimming was affected. These results clearly indicate that motility plays an important role in the pathogenicity of this bacterium.
International
Si
JCR
Si
Title
MICROBIOLOGY-SGM
ISBN
1350-0872
Impact factor JCR
2,841
Impact info
Volume
155
DOI 10.1099/mic.0.022244-0
Journal number
0
From page
434
To page
442
Month
ENERO
Ranking
Participants
  • Autor: Maria Antunez Lamas (UPM)
  • Autor: Emilia Antonia Lopez Solanilla (UPM)
  • Autor: Pablo Rodriguez Palenzuela (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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