Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Epigenetic changes in micropropagated hop plants.
Year:2009
Research Areas
  • Molecular biology
Information
Abstract
Micropropagation isawidelyusedtechniqueinhops(Humulus lupulus L.). However, to thebestofourknowledge,thegeneticandepigeneticstabilityofthemicroplants has neverbeentestedbefore.Inthepresentstudy,twohopaccessionswere established in vitro and micropropagatedfor2years.Thegeneticandepigenetic stability ofthe in vitro plants wasanalyzedwithseveralmoleculartechniques: random amplifiedDNApolymorphism(RAPD),retrotransposonmicrosatellite amplified polymorphism(REMAP),andmethylation-sensitiveamplificationpoly- morphism (MSAP).Nogeneticvariationamongcontrolandtreatedplantswasfound, even after12cyclesofmicropropagation.Epigeneticvariationwasdetected,first, when fieldand in vitro samples werecompared.Nearlya30%ofthedetected fragments presentedthesamepatternofalterationsinallthevitroplants.Second, lower levelsofepigeneticvariationweredetectedamongplantsfromthedifferent subcultures. Partofthisdetectedvariationseemedtobeaccumulatedalongthe12 sequential subculturestested.
International
Si
JCR
Si
Title
JOURNAL OF PLANT PHYSIOLOGY
ISBN
0176-1617
Impact factor JCR
2,437
Impact info
Volume
Journal number
0
From page
234
To page
243
Month
ENERO
Ranking
Participants
  • Participante: M.Angeles Revilla
  • Autor: Rosa Adela Arroyo Garcia (UPM)
  • Participante: Elena L. Peredoa
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
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