Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
Bimolecular Complementation assays to study protein-protein interactions in plants
Year:2009
Research Areas
  • Molecular biology
Information
Abstract
A wide variety of experimental approaches have been developed to study protein-protein interactions in plants, however most of them require disruption of cells or indirect assays of protein detection. The development of sensitive and versatile methods able to detect these interactions in vivo and in homologous tissue and species, is essential to understand protein functions. The Bimolecular Complementation (BiC) either using fluorescent (BiFC) or luminescent (BiLC) compounds provides a direct approach of visualization of molecular interaction in real time, living cells at the subcellular level and under real physiological conditions. BiFC and BiLC procedures are based on the complementation between two fragments of a fluorescent/luminescent protein that can associate when they are brought together by the interaction between proteins fused to each of the fragments. Recent improvements of this technique, the multicolour BiFC approach (mBiFC), have been developed for the simultaneous visualization of multiple protein interactions in the same cell. The combination of fragments of different types of fluorescent proteins makes possible the simultaneous detection of protein complexes in planta. The great potential of this technology to determine biological interactions will be presented.
International
No
Congress
XIII Congress of the Spanish Society for Cell Biology
960
Place
Murcia
Reviewers
No
ISBN/ISSN
9788474011975
Start Date
16/12/2009
End Date
18/12/2009
From page
48
To page
48
XIII Congress of SEBC
Participants
  • Participante: I. HERRERO
  • Autor: M. Isabel Diaz Rodriguez (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Laura Carrillo Gil (UPM)
Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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