Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Localization of connexins in neurons and glía cells of the Helix aspersa suboesophageal brain ganglia by immunocytochemistry
Año:2007
Áreas de investigación
  • Medicina
Datos
Descripción
The aim of this work was to characterize several ionic channels in nervous cells of the suboesophageal visceral, left and right parietal, and left and right pleural brain ganglia complex of the snail Helix aspersa by immunocytochemistry. We have studied the immunostaining reaction for a wide panel of eleven polyclonal antibodies raised against mammal antigens as follows: voltage-gated-Na+ channel; voltage-gated-delayed-rectifier-K+ channel; SK2-small-conductance-Ca2+-dependent-K+ channel apamin sensitive; SK3 potassium channel; charybdotoxin-sensitive voltage-dependent potassium channel; BKCa-maxi-conductance-Ca2+-dependent-K+ channel; hyperpolarization-activated cyclic nucleotide-gated potassium channel 4; G-protein-activated inwardly rectifying potassium channel GIRK2 and voltage-gated-calcium of L, N and P/Q type channels. Our results show positive reaction in neurons, but neither in glia cells nor in processes in the Helix suboesophageal ganglia. Our results suggest the occurrence of molecules in Helix neurons sharing antigenic determinants with mammal ionic channels. The reaction density and distribution of immunoreactive staining within neurons is specific for each one of the antisera tested. The studies of co-localization of immunoreaction, on alternate serial sections of the anterior right parietal ganglion, have shown for several recognized mapped neurons that they can simultaneously be expressed among two and seven different ionic protein channels. These results are considered a key structural support for the interpretation of Helix aspersa neuron electrophysiological activity.
Internacional
Si
JCR del ISI
Si
Título de la revista
HISTOL HISTOPATHOL
ISSN
0950-9232
Factor de impacto JCR
2,007
Información de impacto
Volumen
DOI
Número de revista
0
Desde la página
497
Hasta la página
507
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: A. VERA-GIL
  • Autor: C MARTÍNEZ-CIRIANO
  • Autor: C. MAESTÚ (UPM)
  • autor: J. AISA
  • Autor: M. PES
  • autor: MJ AZANZA
  • Autor: Francisco del Pozo Guerrero (UPM)
  • Autor: A. del Moral
  • Autor: RN PÉREZ-BRUZÓN
  • Autor: M. RASO
  • Autor: C. JUNQUERA
  • Autor: C. PÉREZ CASTEJÓN
  • Autor: JM LAHOZ
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Grupo de Bioingeniería y Telemedicina
  • Departamento: Tecnología Fotónica
S2i 2018 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)