|
Abstract
|
|
|---|---|
| There is some concern about the possible occurrence of somaclonal variation induced by cryopreservation techniques in plants. Morphological variations have been reported after in vitro culture and after cryopreservation. Recent genetic stability studies in cryopreserved chrysanthemum shoot apices have revealed that variation in the regenerated cryopreserved material is not a rare phenomenon and also that the instability can be observed in regenerants from certain pre-culture steps (Martín et al. 2011). Sixteen plants derived from several steps of an encapsulation-dehydration protocol were potted, grown in a green house for two years and used to study their morphological characters and genetic profiles by RAPD markers. Leaf (6), stem (1) and inflorescence/flowers (16) morphometric characters were studied and twelve RAPD primers were employed. Two of the cryopreserved samples showed morphometric differences with respect to other samples according to the principal component and cluster analyses performed, and differences in the RAPD profiles were found in some samples, although these two types of variations were not related. | |
|
International
|
Si |
|
Congress
|
The Society for Low Temperature Biology Symposium 2011 "Preservation of Forest Species" |
|
|
960 |
|
Place
|
Londres (Reino Unido) |
|
Reviewers
|
No |
|
ISBN/ISSN
|
|
|
|
|
|
Start Date
|
06/10/2011 |
|
End Date
|
07/10/2011 |
|
From page
|
|
|
To page
|
|
|
|
|