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Memorias de investigación
Ponencias en congresos:
VIitrification of cryopreserved mint shoot tips as followed by cryo-SEM and DSC
Año:2011
Áreas de investigación
  • Producción vegetal
Datos
Descripción
Several currently successfully applied plant germplasm cryopreservation methods rely on cytoplasm vitrification for storage with almost complete stoppage of both chemical reactions and physical processes and without ice crystal biological damage. These procedures involve a serial of previous steps leading to natural defence induction, water content reduction and overall cytoplasm molecular mobility impairment. A quick cooling step by immersion in liquid nitrogen follows. Mint shoot tips, in different stages of the ?droplet? cryopreservation protocol, were studied both by cold-stage scanning electron microscopy (cryo-SEM) and differential scanning calorimetry (DSC). Results show how cells at intermediate treatment steps develop ice crystals during liquid nitrogen cooling, while specimens whose treatment was completed become vitrified, with no evidence of ice formation. These results perfectly agree with the generally observed plant recuperation after cryopreservation and allow some preliminary conclusions to be extracted, concerning the cryopreservation protocol followed.
Internacional
Si
Nombre congreso
23rd IIR International Congress of Refrigeration
Tipo de participación
960
Lugar del congreso
Praga
Revisores
Si
ISBN o ISSN
978-2-913-149-89-2
DOI
Fecha inicio congreso
21/08/2011
Fecha fin congreso
26/08/2011
Desde la página
1
Hasta la página
7
Título de las actas
Proceedings of the 23rd IIR International Congress of Refrigeration
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Aline Schneider Teixeira (ICTAN-CSIC)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: A.D. Molina-Garcia (ICTAN-CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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