Descripción
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The reconstruction of the cell lineage tree of early zebrafish embryogenesis requires the use of in-vivo microscopy imaging and image processing strategies. Second (SHG) and third harmonic generation (THG) microscopy observations in unstained zebrafish embryos allows to detect cell divisions and cell membranes from 1-cell to 1K-cell stage. In this article, we present an ad-hoc image processing pipeline for cell tracking and cell membranes segmentation enabling the reconstruction of the early zebrafish cell lineage tree until the 1K-cell stage. This methodology has been used to obtain digital zebrafish embryos allowing to generate a quantitative description of early zebrafish embryogenesis with minute temporal accuracy and ?m spatial resolution | |
Internacional
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Si |
Nombre congreso
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IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011) |
Tipo de participación
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960 |
Lugar del congreso
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Chicago, IL USA |
Revisores
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Si |
ISBN o ISSN
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1945-7936 |
DOI
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10.1109/ISBI.2011.5872699 |
Fecha inicio congreso
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30/03/2011 |
Fecha fin congreso
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02/04/2011 |
Desde la página
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1561 |
Hasta la página
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1564 |
Título de las actas
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IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011) |