Memorias de investigación
Research Publications in journals:
Cysteine peptidases and their inhibitors inTetranychus urticae: a comparative genomic approach
Year:2012

Research Areas
  • Natural sciences and health sciences,
  • Molecular biology

Information
Abstract
Background: Cysteine peptidases in the two-spotted spider mite Tetranychus urticae are involved in essential physiological processes, including proteolytic digestion. Cystatins and thyropins are inhibitors of cysteine peptidases that modulate their activity, although their function in this species has yet to be investigated. Comparative genomic analyses are powerful tools to obtain advanced knowledge into the presence and evolution of both, peptidases and their inhibitors, and could aid to elucidate issues concerning the function of these proteins. Results: We have performed a genomic comparative analysis of cysteine peptidases and their inhibitors in T. urticae and representative species of different arthropod taxonomic groups. The results indicate: i) clade-specific proliferations are common to C1A papain-like peptidases and for the I25B cystatin family of inhibitors, whereas the C1A inhibitors thyropins are evolutionarily more conserved among arthropod clades; ii) an unprecedented extensive expansion for C13 legumain-like peptidases is found in T. urticae; iii) a sequence-structure analysis of the spider mite cystatins suggests that diversification may be related to an expansion of their inhibitory range; and iv) an in silico transcriptomic analysis shows that most cathepsin B and L cysteine peptidases, legumains and several members of the cystatin family are expressed at a higher rate in T. urticae feeding stages than in embryos. Conclusion: Comparative genomics has provided valuable insights on the spider mite cysteine peptidases and their inhibitors. Mite-specific proliferations of C1A and C13 peptidase and I25 cystatin families and their overexpression in feeding stages of mites fit with a putative role in mite?s feeding and could have a key role in its broad host feeding range.
International
Si
JCR
Si
Title
BMC Genomics
ISBN
1471-2164
Impact factor JCR
4,206
Impact info
Datos JCR del año 2010
Volume
13
10.1186/1471-2164-13-307
Journal number
From page
307
To page
319
Month
SIN MES
Ranking
Participants
  • Autor: Estrella Santamaria Universidad de Onatario-Canada
  • Participante: Pedro Hernandez-Crespo CIB-CSIC
  • Autor: Felix Ortego CIB-CSIC
  • Autor: Vojislava Grbic Universidad de Onatrio-Canada
  • Autor: Miodrag Grbic Universidad de Onatrio-Canada
  • Autor: M. Isabel Diaz Rodriguez UPM
  • Autor: Manuel Martinez Muñoz UPM

Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología