Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
ESPINA: a tool for the automated segmentation and counting of synapses in large stacks of electron microscopy images
Año:2011
Áreas de investigación
  • Morfología humana,
  • Informática aplicada,
  • Interactividad,
  • Software
Datos
Descripción
The synapses in the cerebral cortex can be classified into two main types, Gray?s type I and type II, which correspond to asymmetric (mostly glutamatergic excitatory) and symmetric (inhibitory GABAergic) synapses, respectively. Hence, the quantification and identification of their different types and the proportions in which they are found, is extraordinarily important in terms of brain function. The ideal approach to calculate the number of synapses per unit volume is to analyze 3D samples reconstructed from serial sections. However, obtaining serial sections by transmission electron microscopy is an extremely time consuming and technically demanding task. Using focused ion beam/scanning electron microscope microscopy, we recently showed that virtually all synapses can be accurately identified as asymmetric or symmetric synapses when they are visualized, reconstructed, and quantified from large 3D tissue samples obtained in an automated manner. Nevertheless, the analysis, segmentation, and quantification of synapses is still a labor intensive procedure. Thus, novel solutions are currently necessary to deal with the large volume of data that is being generated by automated 3D electron microscopy. Accordingly, we have developed ESPINA, a software tool that performs the automated segmentation and counting of synapses in a reconstructed 3D volume of the cerebral cortex, and that greatly facilitates and accelerates these processes.
Internacional
Si
JCR del ISI
Si
Título de la revista
Frontiers in Neuroanatomy
ISSN
1662-5129
Factor de impacto JCR
Información de impacto
Volumen
5
DOI
10.3389/fnana.2011.00018
Número de revista
18
Desde la página
1
Hasta la página
8
Mes
MARZO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Juan Morales Del Olmo (UPM)
  • Autor: Lidia Alonso-Nanclares (CSCI)
  • Autor: José Rodrigo-Rodríguez (CSIC)
  • Autor: Javier De Felipe Oroquieta (UPM)
  • Autor: Angel Rodriguez Mtnez.de Bartolome (UPM)
  • Autor: Angel Merchan Perez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Arquitectura y Tecnología de Sistemas Informáticos
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)