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Memorias de investigación
Artículos en revistas:
The Role of Evolutionary Intermediates in the Host Adaptation of Canine Parvovirus
Año:2012
Áreas de investigación
  • Virología,
  • Biodiversidad
Datos
Descripción
The adaptation of viruses to new hosts is a poorly understood process likely involving a variety of viral structures and functions that allow efficient replication and spread. Canine parvovirus (CPV) emerged in the late 1970s as a host-range variant of a virus related to feline panleukopenia virus (FPV). Within a few years of its emergence in dogs, there was a worldwide replacement of the initial virus strain (CPV type 2) by a variant (CPV type 2a) characterized by four amino acid differences in the capsid protein. However, the evolutionary processes that underlie the acquisition of these four mutations, as well as their effects on viral fitness, both singly and in combination, are still uncertain. Using a comprehensive experimental analysis of multiple intermediate mutational combinations, we show that these four capsid mutations act in concert to alter antigenicity, cell receptor binding, and relative in vitro growth in feline cells. Hence, host adaptation involved complex interactions among both surface-exposed and buried capsid mutations that together altered cell infection and immune escape properties of the viruses. Notably, most intermediate viral genotypes containing different combinations of the four key amino acids possessed markedly lower fitness than the wild-type viruses.
Internacional
Si
JCR del ISI
Si
Título de la revista
Journal of Virology
ISSN
0022-538X
Factor de impacto JCR
5,402
Información de impacto
Volumen
86
DOI
10.1128/JVI.06222-11
Número de revista
Desde la página
1514
Hasta la página
1521
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: K. M. Stucker
  • Autor: Jesus Israel Pagan Muñoz (UPM)
  • Autor: J. O. Cifuente
  • Autor: J. T. Kaelber
  • Autor: T. D. Lillie
  • Autor: S. Hafenstein
  • Autor: E. C. Holmes
  • Autor: C. R. Parrish
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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