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Memorias de investigación
Artículos en revistas:
New Carbosilane Polymers with Interacting Ferrocenes as Support and Bioelectrocatalysts of Oxidases to Develop Versatile and Specific Amperometric Biodevice
Año:2012
Áreas de investigación
  • Macromoléculas y polímeros
Datos
Descripción
In this work, the bioelectrocatalytical properties and kinetic characteristics of new oxidase amperometric biosensors based on two different ferrocene functionalized carbosilane polymers, polydiallylmethylsilane (PDAMS) and polymethyldiundecenylsilane (PMDUS) are described. In the development of these biodevices, glucose oxidase has been used as example of oxidase enzyme, and two different immobilization procedures have been studied. The polymer-modified electrodes act as efficient transducers for glucose sensing in anodic and cathodic aerobic conditions and also in anodic anaerobic conditions, and this fact turns them into useful devices for a wide field of applications. PMDUS has shown to be the bioelectrocatalyst with best kinetic and analytical properties in aerobic media while PDAMS was better in anaerobic conditions. The best aerobic biosensor developed displayed a strictly linear range from 0 to 3.0 mM, a detection limit of 7.8 ?M and a response time less than 2 s in an ascorbate interference free work potential interval. The apparent Michaelis?Menten constant was calculated to be 1.36 mM according to the Lineweaver?Burk equation.
Internacional
Si
JCR del ISI
Si
Título de la revista
Applied Biochemistry And Biotechnology
ISSN
0273-2289
Factor de impacto JCR
1,943
Información de impacto
Volumen
168
DOI
Número de revista
Desde la página
1778
Hasta la página
1791
Mes
SIN MES
Ranking
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Participantes
  • Autor: Jose Losada Del Barrio (UPM)
  • Autor: Maria Pilar Garcia Armada (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Polímeros, Caracterización y Aplicaciones
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