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Memorias de investigación
Ponencias en congresos:
IDENTIFICATION AND ANALYSIS OF A NICKEL-INDUCIBLE CATION DIFFUSION FACILITATOR-NICOT COMBINED SYSTEM IN RHIZOBIUM LEGUMINOSARUM BV. VICIAE
Año:2012
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
Analysis of Rhizobium leguminosarum bv. viciae strain 3841 genome sequence revealed a genetic system (RL1175 through RL1178), potentially involved in nickel homeostasis. The predicted RL1175 gene product shows sequence similarity to cation diffusion facilitator proteins, whereas RL1178 encodes a high-affinity nickel uptake transporter of the NiCoT family (nickel-cobalt transport) and the RL1176 gene product is an NreA-like nickel- responsive regulator. The confluence of uptake and efflux transport proteins potentially linked in the same genetic system resembles the organization of the ncrABC and nreBAC operons from Serratia marcenses and Legionella pneumophila, involved in nickel and cobalt resistance. Gene expression analysis using promoter fusions to lacZ and qRT-PCR assays revealed that the identified R. leguminosarum genes are organized as a single operon whose transcription is strongly induced by nickel, but not cobalt, ions in free-living cultures, as well as in pea bacteroid cells. Generation of mutants affected in this system is in progress and will hopefully help to define the role of the interplay between metal uptake and metal efflux systems in nickel resistance.
Internacional
Si
Nombre congreso
10th European Nitrogen Fixation Conference
Tipo de participación
960
Lugar del congreso
Munich, Alemania
Revisores
Si
ISBN o ISSN
00000-00000
DOI
Fecha inicio congreso
02/09/2012
Fecha fin congreso
05/09/2012
Desde la página
23
Hasta la página
23
Título de las actas
Abstract book
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Rosa Isabel Prieto Carbajo (UPM)
  • Autor: Laura Rubio Sanz (UPM)
  • Autor: Silvia Burruezo (Universidad Politecnica de Madrid)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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