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Descripción
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| Dehydrins are thought to play an essential role in the response, acclimation and tolerance to different abiotic stresses, such as cold and drought. Due to their putative functions in the response to stress, dehydrins have been often used as candidate genes in studies on population variability and local adaptation to environmental conditions, especially in Pinus pinaster or Pinus halepensis. However, little is still known regarding dehydrin gene family from the genetically depauperate Pinus pinea, a well-adapted conifer to the high temperatures and drought characteristic of Mediterranean climates. Based on the sequences corresponding to dehydrins described in previous work with pines we have isolated eight different dehydrins from stone pine. We have obtained their genomic and mRNA sequences and identified their intron/exon structure. We have also classified them according to their conserved segments in the deduced amino acid sequences, and predicted their secondary structure and disordered regions. Quantitative RT-PCR analysis of their expression pattern in needles, stems and roots during a severe and prolonged drought stress showed a severe increase of transcripts levels in six of them, especially when the water potential reach values around of -3MPa at 40 day without irrigation. Comparison between the expression patterns obtained in Pinus pinaster and Pinus pinea shows notable differences in levels of transcripts, especially in aerial parts where Pinus pinea shows remarkable higher inductions. | |
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Internacional
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Si |
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Nombre congreso
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5th International Conference on Mediterranean Pines (medpine5) |
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Tipo de participación
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960 |
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Lugar del congreso
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Revisores
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Si |
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ISBN o ISSN
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00-0000-000-0 |
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DOI
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Fecha inicio congreso
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22/09/2014 |
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Fecha fin congreso
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26/09/2014 |
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Desde la página
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77 |
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Hasta la página
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77 |
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Título de las actas
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Book of abstracts |