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Ponencias en congresos:
Native Bradyrhizobium strains play an essential role in the conservation of the endangered Lupinus mariae-josephae endemism in Eastern Spain
Áreas de investigación
  • Ciencias biológicas,
  • Ecología
Lupinus mariae-josephae (Lmj) is a lupin endemism of Valencia Region (Spain), where it grows in a few defined patches of a few hundred of plants each. The L. mariae-josephae, contrary to all hitherto described Old- and New-World lupines, which are preferentially adapted to acid soils, thrives on alkaline soils of the ?terra rossa? type. Efforts directed towards controlled plant reproduction have been unsuccessful. Greenhouse experiments using L. mariae-josephae trap-plants showed that Lmj establish a root nodule symbiosis with specific bradyrhizobia isolated from soil of the plant patches and that its concentration is very low outside the plant spots. These bradyrhizobia are absent in other ?terra rossa? or alkaline red soils from Iberian Peninsula and Balearic Island. The characterization of Lmj-nodulating bacteria from Valencia soils have recently been undertaken and studies from our research group concluded that they belong to a new Bradyrhizobiun lineage unrelated to B. canariense or B. japonicum. Based on genetic, genomic, proteomic and phenotypic data a new species, named Bradyrhizobium valentinum (LmjM3T), was defined. We hypothesized that the almost absence of Lmj-nodulating bacteria in soils might contribute to the lack of success in reproducing plants for conservation purposes. To investigate this possibility, we carried out two planting experiments in consecutive years under natural conditions in areas with edapho-climatic characteristics identical to those sustaining natural Lmj populations. Two strains, LmjM3T and LmjC, were selected as inoculum for seed coating based on its higher efficiency to nodulate and fix N2 with their cognate host in greenhouse assays. The results of these experiments clearly showed that a successful reproductive cycle was absolutely dependent on seedling inoculation with effective bradyrhizobia, and optimal performance was observed in plants inoculated with LmjC. Our results define conditions for L. mariae-josephae conservation, and highlight the rhizobia symbiosis as a trait to be considered as strategy for conservation of endangered legume species.
Nombre congreso
11th European Nitrogen Fixation Conference
Tipo de participación
Lugar del congreso
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Título de las actas
Libro de resúmenes del congreso
Esta actividad pertenece a memorias de investigación
  • Autor: David Ricardo Duran Wendt (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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