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Memorias de investigación
Artículos en revistas:
Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips
Año:2015
Áreas de investigación
  • Producción vegetal
Datos
Descripción
This study compared the effect of the cryopreservation protocol, the genotype and the plant growth regulators used in the recovery media on the genetic stability of mint shoot tips. Shoot tips of two micropropagated genotypes of mint were cryopreserved either using an encapsulation-dehydration or droplet-vitrification protocol. Three recovery media were tested, all of them MS based although with different plant growth regulators: (1) 0.5 mg L-1 6-benzylaminopurine (BAP); (2) 0.5 mg L-1 6-dimethylallylamino-purine (2iP) + 0.1 mg L-1 a-naphthalene acetic acid (NAA); and (3) 0.5 mg L-1 BAP ? 0.1 mg L-1 NAA. DNA was extracted from three different types of samples: leaves from shoots, callus at the base of shoots and callus. RAPD markers were used to assess the genetic stability. One of the genotypes, ?MEN 198?, showed higher percentage of stable samples than the other one, ?MEN 186? (97 vs. 42 %; considering all media, protocol and type of explant). The material recovered after droplet-vitrification showed higher stability compared to the encapsulation-dehydration recovered samples: 99 versus 87 % in ?MEN 198?, and 80 versus 24 % in ?MEN 186?. There were not large differences in the genetic stability obtained from the three types of samples in ?MEN 196? (85?100 %), while in ?MEN 186? calli were more unstable (53, 44 and 30 % stability for leaves, basal callus and callus, respectively). The effect of the recovery medium on the stability of the samples was noticeable in the more unstable types of explants but not in leaves.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Cell Tissue And Organ Culture
ISSN
0167-6857
Factor de impacto JCR
2,612
Información de impacto
Datos JCR del año 2013
Volumen
122
DOI
10.1007/s11240-015-0760-0
Número de revista
Desde la página
185
Hasta la página
195
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: Carolina Kremer (UPM)
  • Autor: Iván González (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Departamento: Biotecnología - Biología Vegetal
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