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Memorias de investigación
Artículos en revistas:
Isolation of recombinant antibody fragments (scFv) by phage display technology for detection of almond allergens in food products
Año:2015
Áreas de investigación
  • Tecnología de alimentos
Datos
Descripción
Tree nut allergies are considered an important health issue in developed countries. To comply with the regulations on food labeling, reliable allergen detection methods are required. In this work we isolated almond-specific recombinant antibody fragments (scFv) from a commercial phage display library bypassing the use of live animals, hence being consistent with the latest policies on animal welfare. To this end an iterative selection procedure employing the Tomlinson I phage display library and a crude almond protein extract was carried out. Two different almond-specific scFv (named PD1F6 and PD2C9) were isolated after two rounds of biopanning, and an indirect phage ELISA was implemented to detect the presence of almond protein in foodstuffs. The isolated scFvs demonstrated to be highly specific and allowed detection of 40 ng mL?1 and 100 ng mL?1 of raw and roasted almond protein, respectively. The practical detection limit of the assay in almond spiked food products was 0.1 mg g?1 (110e120 ppm). The developed indirect phage ELISA was validated by analysis of 92 commercial food products, showing good correlation with the results obtained by a previously developed real-time PCR method for the detection of almond in foodstuffs. The selected phage clones can be affinity maturated to improve their sensitivity and genetically engineered to be employed in different assay formats.
Internacional
Si
JCR del ISI
Si
Título de la revista
Food Control
ISSN
0956-7135
Factor de impacto JCR
2,806
Información de impacto
Volumen
54
DOI
Número de revista
Desde la página
322
Hasta la página
330
Mes
SIN MES
Ranking
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Participantes
  • Autor: Satyabrata Ghosh . (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: LPF-TAGRALIA: Técnicas Avanzadas en Agroalimentación
  • Departamento: Ingeniería Agroforestal
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