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Descripción
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| Some rhizobia are able to effectively nodulate several legume plants, which constitute a crossinoculation group. In the case of Rhizobium leguminosarum bv viciae the cross inoculation group includes plants belonging to genera Pisum, Lens, Lathyrus, and Vicia. It has been shown that different hosts within this group modulate the expression of rhizobial traits in a host-specific manner, suggesting that different plants might impose different environments for the endosymbiont. For instance, when pea and lentil plants interact with the same R. leguminosarum bv viciae strain, significant differences occur in the symbiotic expression of hydrogenase (1), and also in nickel input and efflux (2,3). These data indicate that bacteria adapt differently to the conditions provided by each host. A relevant aspect of the symbiotic environment within the nodule is the presence of nodule-specific cysteine-rich (NCR) peptides produced by the plant and exported into the bacteroids (4), a phenomenon widely extended in legume plants from the IRLC (Inverted Repeat- Lacking Clade) group (5). In this communication we present a proteomics approach to study the differential expression in bacteroids induced by R. leguminosarum strain UPM791 in pea and lentil plants. In each case, over 800 bacterial proteins were identified through LC-mass spectrometry analysis. Differences between bacteroids from both hosts affected a surprisingly high number of proteins. These differences are currently being quantified through iTRAQ labelling. Plant-derived NCR peptides were also identified in bacteroid extracts from both legumes. The identity of these peptides is being determined through complementary transcriptomic analysis. Preliminary data indicate that pea and lentil plants deliver to the bacteroids a mixture of common and host-specific NCR peptides | |
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Internacional
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No |
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Nombre congreso
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12th European Nitrogen Fixation Conference |
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Tipo de participación
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970 |
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Lugar del congreso
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Budapest |
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Revisores
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Si |
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ISBN o ISSN
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978-615-5270-26-0 |
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DOI
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Fecha inicio congreso
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25/08/2016 |
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Fecha fin congreso
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28/08/2016 |
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Desde la página
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262 |
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Hasta la página
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262 |
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Título de las actas
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12th European Nitrogen Fixation Conference |