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Memorias de investigación
Conferencias:
Expression of Nitrogenase Component Proteins is Eukaryotes. The nitrogen nutrition of plants.
Año:2016
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
The extensive use of synthetic N fertilizers in developed countries poses enormous environmental threats that must be addressed. The awareness that biological N2 fixation can be used as alternative to the synthetic N fertilizers in the implementation of modern sustainable agricultural practices is an underlying driving force for nitrogenase studies. An ambitious challenge of plant biotechnology is to increase cereal crop productivity by engineering plants to fix their own nitrogen, i.e. by functional expression of bacterial N2 fixation (nif) genes in the plant. Two identified barriers have traditionally impaired this approach: the known sensitivity of nitrogenase to O2 (the by-product of plant photosynthesis) and the genetic/biochemical complexity of nitrogenase biosynthesis. Balanced expression of at least 9 nif gene is required to mature nitrogenase structural polypeptides into their catalytically active forms. Both nitrogenase components, as well as most proteins required for the assembly of their metal clusters, are very sensitive to O2. Here we present data demonstrating that the mitochondrial matrix provides a low O2 environment appropriate for the assembly and the activity of nitrogenase components. In addition, a combined synthetic biology and biochemical complementation approach is being used to tackle the problem of nitrogenase genetic complexity. Advances towards expressing a full nitrogenase complex in eukaryotic cells will be presented.
Internacional
Si
ISSN o ISBN
0000000000
Entidad relacionada
EMBO Conference: The nitrogen nutrition of plants
Nacionalidad Entidad
Sin nacionalidad
Lugar del congreso
Montpellier
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Gema Lopez Torrejon (UPM)
  • Autor: Elena Caro Bernat (UPM)
  • Autor: Nils Stefan Buren (UPM)
  • Autor: Carlos Echavarri Erasun (UPM)
  • Autor: Emilio Jimenez Vicente (UPM)
  • Autor: José María Buesa Galiano (UPM)
  • Autor: Mónica Navarro Rodríguez (UPM)
  • Autor: Ana Perez Gonzalez (UPM)
  • Autor: Marcel Veldhuizen . (UPM)
  • Autor: Luis Manuel Rubio Herrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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