|
Descripción
|
|
|---|---|
| The plant cell wall is one of the first defensive barriers that pathogens need to overcome to successfully colonize plant tissues. Plant cell wall is considered a dynamic structure that regulates both constitutive and inducible defense mechanisms. The wall is a potential source of a diverse set of damage-associated molecular patterns (DAMPs), which are signalling molecules that trigger immune responses. However, just a few active wall ligands, like oligogalacturonic acids (OGs), have been characterised so far. To identify additional wall-derived DAMPs, we obtained different plant wall fractions and tested their capacity to trigger immune responses using a calcium read-out system. To characterise the active DAMPs structures present in these fractions, we applied Glycome Profiling, a technology that uses a large and diverse set of specific monoclonal antibodies against wall carbohydrate ligands. The methods describe here can be used in combination with other biochemical approaches to identify and purify new plant cell wall DAMPs. | |
|
Internacional
|
Si |
|
DOI
|
10.1007/978-1-4939-6859-6_2 |
|
Edición del Libro
|
|
|
Editorial del Libro
|
Springer Science+Business Media LLC |
|
ISBN
|
978-1-4939-6859-6 |
|
Serie
|
|
|
Título del Libro
|
Plant Pattern Recognition Receptors |
|
Desde página
|
13 |
|
Hasta página
|
23 |