Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
DNA demethylases promote bud break in poplar
Año:2016
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Ciencias de la tierra,
  • Biología molecular, celular y genética,
  • Ciencias biológicas,
  • Biología,
  • Ingeniería biológica,
  • Biología vegetal, animal y ecología,
  • Agricultura
Datos
Descripción
Epigenetic mechanisms have been proposed to synchronize dormancy-growth transition in trees by generating reversible patterns of epigenetic marks (1, 2). Dormancy consists of growth cessation through the arrest of meristem activity and consequent transformation of the apical meristem into a dormant apical bud. A progressive demethylation of dormant apical bud has been suggested to play a role during the reactivation of growth, bud break (3, 4). However, so far no chromatin remodelers have been functionally related to bud break. In this work we report the identification of two poplar DEMETER-Like genes: PtaDML8 and PtaDML10. Our RT-PCR analyses showed that these genes are highly induced during bud break. Phenological assays showed that PtaDML8-10 knockdown plants (KD) have a delayed bud break. Transcriptome analyses revealed that these KD plants have altered biological processes such as cellular metabolic process, photosynthesis, ribosome biogenesis and response to light or temperature stimulus. We have also identified DMRs (differentially methylated region) in KDs vs. WT plants at bud break. This work shows, for the first time, that PtaDML8 and PtaDML10 are involved in active DNA demethylation required for bud break and growth resume.
Internacional
Si
Nombre congreso
Workshop New Frontiers in Plant Biology
Tipo de participación
970
Lugar del congreso
Centro de Biotecnología y Genómica de Plantas, Pozuelo de Alarcón, Madrid, España
Revisores
Si
ISBN o ISSN
0000-0000
DOI
Fecha inicio congreso
15/06/2016
Fecha fin congreso
17/06/2016
Desde la página
10
Hasta la página
10
Título de las actas
DNA demethylases promote bud break in poplar
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Daniel Conde Rodriguez (UPM)
  • Autor: Alicia Moreno Cortes (UPM)
  • Autor: Anne-Laure Le Gac
  • Autor: Christopher Dervinis
  • Autor: Jose Manuel Ramos Sanchez (UPM)
  • Autor: Matias Kirst
  • Autor: Stéphane Maury
  • Autor: Mariano Manuel Perales (UPM)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Isabel Marta Allona Alberich (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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