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Descripción
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| Necrotrophic fungal pathogens cause major pre- and post-harvest diseases in numerous crops resulting in important economic losses. Plant colonization success by necrotrophic fungi relies on sophisticated molecular mechanisms. The identification of fungal determinants required for necrotrophy will contribute to develop novel crop protection strategies (Wang et al. 2014). Plectosphaerella cucumerina (Pc) is an ascomycete ubiquitous fungus which is adapted to colonise different plant hosts (Ramos et al, 2013). We have characterised different Pc isolates that display distinct types of interactions with the model plant Arabidopsis thaliana: PcBMM, a pathogenic (adapted) isolate; Pc2127, a non-pathogenic (non-adapted) isolate in wild-type plants; and Pc0831, an endophytic isolate identified in wild populations of A. thaliana in central Spain, that colonises wild-type plants without causing disease symptoms (García et al., 2013). We have sequenced, assembled and compared the genomes of these three Pc isolates to identify genomic signatures associated to the different lifestyles. Though these isolates have similar gene arsenals and share more than 90% of gene families, comparative transcriptomic analyses identified gene families differentially regulated in planta (Arabidopsis thaliana) vs in vitro, including a repertoir of PcBMM genes encoding potenial effectors. The characterization of in planta expressed specific gene families from the Pc isolates and the determination of the their contribution to Pc lifestyle will be presented. | |
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Internacional
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Si |
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Nombre congreso
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Workshop New Frontiers in Plant Biology |
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Tipo de participación
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970 |
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Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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15/06/2016 |
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Fecha fin congreso
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17/06/2016 |
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Desde la página
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25 |
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Hasta la página
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25 |
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Título de las actas
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Comparative and functional genomics of Plectosphaerella cucumerina isolates with different life styles |