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Memorias de investigación
Ponencias en congresos:
Medicago truncatula Ferroportin2 (MtFPN2) is a nodule- specific iron exporter
Año:2017
Áreas de investigación
  • Biología molecular, celular y genética,
  • Microbiología
Datos
Descripción
Legumes are able to use atmospheric nitrogen and convert it to ammonia through a partnership with endosymbiotic rhizobia. This symbiotic nitrogen fixation (SNF) occurs in root nodules formed with rhizobia. SNF requires different transition metals, notably iron, as essential cofactors of multiple proteins involved in this process (leghemoglobin, nitrogenase?). Previous studies in our laboratory showed that iron is driven from vascular bundles to the aploplast of the infection zone of the nodule (1). Transcriptomic studies in Medicago truncatula nodules showed that MtFPN2 is specific to the nodule. Within this organ, promoter::gus fusions and immunolocalization showed that it was located in the nodule vasculature and in some intracellular compartment in rhizobia-infected cells. Yeast complementation assays in several metal transporter mutants, indicated that MtFPN2 was able to transport iron and manganese out of the cytosol, either into an organelle or across the plasma membrane. Phenotypical characterization of two Tnt1-insertion mutant alleles (fpn2-1 and fpn2-2) revealed that MtFPN2 was important for SNF, as both lines presented a significantly lower nitrogenase activity than wild-type plants.
Internacional
Si
Nombre congreso
International Congress on Nitrogen Fixation
Tipo de participación
970
Lugar del congreso
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
03/09/2017
Fecha fin congreso
07/09/2017
Desde la página
156
Hasta la página
156
Título de las actas
International Congress on Nitrogen Fixation
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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