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Abstract
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| Nitrogenase, a key enzyme in Symbiotic Nitrogen Fixation (SNF), requires the presence of molybdenum in its active center, as part of the Iron-Molybdenum-cofactor. The host plant has to deliver this metal to the bacteroids, inside the root nodules, in order to synthesize the cofactor. In legumes, transporters mediating molybdenum transport have not been identified so far. In model legume Medicago truncatula we have identified a nodule-specific member of molybdate transporter family MOT1(1), MtMOT1.3. Heterologous expression in Saccharomyces cerevisae reveals that MtMOT1.3 transports molybdate into the cytosol. Imnunolocalization studies show that MtMOT1.3 is expressed in the plasma membrane of nodule cells. A MtMOT1.3 knockout mutant exhibited reduced nitrogenase activity and impaired growth, compared to the wild type under symbiotic conditions. The mutant phenotype was restored by reintroducing a functional copy of MtMOT1.3 or increasing molybdate concentrations in the nutritive solution. No differences in phenotype were observed between WT and mot1.3-1 plants when adding an assimilable nitrogen source. These results indicate the important role of MtMOT1.3 in molybdenum homeostasis in M. truncatula nodules. | |
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International
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No |
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Congress
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Reunión de la Sociedad Española de Fisiología Vegetal |
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960 |
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Place
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Reviewers
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Si |
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ISBN/ISSN
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0000000000 |
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Start Date
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26/06/2017 |
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End Date
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29/06/2017 |
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From page
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20 |
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To page
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20 |
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Reunión de la Sociedad Española de Fisiología Vegetal |