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Abstract
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| Legumes are able to use atmospheric nitrogen and convert it to ammonia through a partnership with endosymbiotic rhizobia. This symbiotic nitrogen fixation (SNF) occurs in root nodules formed with rhizobia. SNF requires different transition metals, notably iron, as essential cofactors of multiple proteins involved in this process (leghemoglobin, nitrogenase?). Previous studies in our laboratory showed that iron is driven from vascular bundles to the aploplast of the infection zone of the nodule (1). Transcriptomic studies in Medicago truncatula nodules showed that MtFPN2 is specific to the nodule. Within this organ, promoter::gus fusions and immunolocalization showed that it was located in the nodule vasculature and in some intracellular compartment in rhizobia-infected cells. Yeast complementation assays in several metal transporter mutants, indicated that MtFPN2 was able to transport iron and manganese out of the cytosol, either into an organelle or across the plasma membrane. Phenotypical characterization of two Tnt1-insertion mutant alleles (fpn2-1 and fpn2-2) revealed that MtFPN2 was important for SNF, as both lines presented a significantly lower nitrogenase activity than wild type plants. | |
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International
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No |
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Congress
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Reunión de la Sociedad Española de Fisiología Vegetal |
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970 |
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Place
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Reviewers
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Si |
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ISBN/ISSN
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0000000000 |
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Start Date
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26/06/2017 |
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End Date
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29/06/2017 |
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From page
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214 |
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To page
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214 |
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Reunión de la Sociedad Española de Fisiología Vegetal |