Memorias de investigación
Artículos en revistas:
Volume electron microscopy of the distribution of synapses in the neuropil of the juvenile rat somatosensory cortex.
Año:2018
Áreas de investigación
  • Ciencias naturales y ciencias de la salud,
  • Ingenierías
Datos
Descripción
Knowing the proportions of asymmetric (excitatory) and symmetric (inhibitory) synapses in the neuropil is critical for understanding the design of cortical circuits. We used focused ion beam milling and scanning electron microscopy (FIB/SEM) to obtain stacks of serial sections from the six layers of the juvenile rat (postnatal day 14) somatosensory cortex (hindlimb representation). We segmented in three-dimensions 6184 synaptic junctions and determined whether they were established on dendritic spines or dendritic shafts. Of all these synapses, 87?94% were asymmetric and 6?13% were symmetric. Asymmetric synapses were preferentially located on dendritic spines in all layers (80?91%) while symmetric synapses were mainly located on dendritic shafts (62?86%). Furthermore, we found that less than 6% of the dendritic spines establish more than one synapse. The vast majority of axospinous synapses were established on the spine head. Synapses on the spine neck were scarce, although they were more common when the dendritic spine established multiple synapses. This study provides a new large quantitative dataset that may contribute not only to the knowledge of the ultrastructure of the cortex, but also towards defining the connectivity patterns through all cortical layers.
Internacional
Si
JCR del ISI
Si
Título de la revista
Brain Structure & Function
ISSN
1863-2653
Factor de impacto JCR
4,231
Información de impacto
Volumen
223
DOI
10.1007/s00429-017-1470-7
Número de revista
1
Desde la página
77
Hasta la página
90
Mes
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Participantes
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Tecnologías para Ciencias de la Salud
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB