Observatorio de I+D+i UPM

Descripción
Semi-selective enrichment, followed by PCR screening, resulted in the successful direct isolation of fast-growing Rhizobia from a dryland agricultural soil. Over 50% of these isolates belong to the genus Neorhizobium, as concluded from partial rpoB and near-complete 16S rDNA sequence analysis. Further genotypic and genomic analysis of five representative isolates confirmed that they form a coherent group within Neorhizobium, closer to N. galegae than to the remaining Neorhizobium species, but clearly differentiated from the former, and constituting at least one new genomospecies within Neorhizobium. All the isolates lacked nod and nif symbiotic genes but contained a repABC replication/maintenance region, characteristic of rhizobial plasmids, within large contigs from their draft genome sequences. These repABC sequences were related, but not identical, to repABC sequences found in symbiotic plasmids from N. galegae, suggesting that the non-symbiotic isolates have the potential to harbor symbiotic plasmids. This is the first report of non-symbiotic members of Neorhizobium from soil.
Internacional	Si
JCR del ISI	Si
Título de la revista	Peerj
ISSN	2167-8359
Factor de impacto JCR	2,177
Información de impacto	Datos JCR del año 2016
Volumen
DOI	10.7717
Número de revista
Desde la página	6
Hasta la página	:e4776
Mes	SIN MES
Ranking

Esta actividad pertenece a memorias de investigación

• Autor: Amalia Soenens Martínez de Murguia (UPM)
• Autor: Juan Imperial Ródenas (UPM)

• Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL