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Descripción
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| Nitrogen is an essential macronutrient whose availability in soil is crucial for plant growth and crop productivity. Nitrate is the main source of inorganic nitrogen for land plants. However, it is usually limiting in the majority of the ecosystems, due to a variety of biotic and abiotic factors like microbial consumption, water leaching and soil erosion. Besides a macronutrient, nitrate is also a signal molecule that regulates the expression of a variety of genes that modulate many aspects of metabolism, growth and development. Here we identify the roles of ZF1 and ZF3, Arabidopsis DOF transcription factors in nitrate responses. Loss-of-function null mutant zf3-1 and zf1R exhibit nitrate-dependent root phenotypes, whereas ZF1 and ZF3 overexpression lines showed increased biomass and altered root architecture. Transcriptomic analyses of overexpressor lines indicated that both factors regulate the expression an important set of carbon and nitrogen regulated genes. Consistently, metabolite profiling disclosed that the total amount of key nitrogen metabolites including glutamate and glutamine were higher in ZF1/3-overexpressing plants in N-sufficient and limiting conditions. By contrast, zf3-1 and zf1R mutants exhibited lower amounts of those metabolites. In tomato, the overexpression of ZF1 and ZF3 genes modified amino acid and sugar content, improving biomass and yield. These results identify ZF1 and ZF3 as important regulatory factors of the nitrate response in Arabidopsis, suggesting that they are potential targets for improving nutrient use efficiency in crops. | |
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Internacional
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Si |
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Nombre congreso
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XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |
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Tipo de participación
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970 |
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Lugar del congreso
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Salamanca |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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04/07/2018 |
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Fecha fin congreso
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06/07/2018 |
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Desde la página
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168 |
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Hasta la página
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168 |
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Título de las actas
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XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |