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Descripción
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| To determine the compatibility of use of the nucleopolyhedroviruses (NPVs) of Spodoptera exigua (SeMNPV) and Autographa californica (AcMNPV) with the neuropteran predator Chrysoperla carnea, laboratory assays with last instar larvae were developed. Four diets composed the treatments: (1) Ephestia kuehniella + Artemia spp. eggs (control 1), (2) healthy L2 S. exigua larvae (control 2), (3) SeMNPV infected L2 S. exigua larvae, (4) AcMNPV infected L2 S. exigua larvae. Spodoptera exigua newly molted L2 larvae were infected via droplets ingestion with the LC95 of SeMNPV (specific NPV of S. exigua) and the LC95 of AcMNPV (wide range NPV of Noctuidae) respectively, and offered ad libitum 3 days post inoculation to L3 C. carnea larvae until pupation. The parameters evaluated were: pupation, weight of pupae, adult emergence, sex ratio, fecundity, egg hatching, adult lifespan, viability of the offspring and presence of occlusion bodies (OBs) in the meconium. LC95 of both NPVs for L2 S. exigua larvae were previously calculated with a PROBIT analysis (SeMNPV: 1.9x105 OBs/ml; AcMNPV: 1.6x106 OBs/ml). There was a delay in the pupation of C. carnea larvae treated with both virus as well as a slight reduction of the pupal weight. No differences were found on the adult emergence. When reproduction parameters were assessed, some differences between treatments were detected. Our findings are relevant for the combined use of virus-based insecticides and natural enemies of lepidopteran pests. | |
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Internacional
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Si |
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Nombre congreso
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International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology &17th Meeting of the IOBC-WPRS Working Group Microbial and Nematode Control of Invertebrate Pests |
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Tipo de participación
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970 |
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Lugar del congreso
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valencia |
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Revisores
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Si |
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ISBN o ISSN
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000-00-00-00000-0 |
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DOI
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Fecha inicio congreso
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28/07/2019 |
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Fecha fin congreso
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01/08/2019 |
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Desde la página
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125 |
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Hasta la página
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125 |
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Título de las actas
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Programme and Abstracts |