Descripción
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During last years of the 20th century, oak decline has been reported in Spain due to several pathogens and/or abiotic factors. One of the causal agents of such decline has been identified as the bacterial phytopathogen Brenneria quercina. To investigate the bacterial variability of this pathogen from different Spanish oak forests, a collection of bacterial isolates from several geographic locations and from different oak species was analysed by sequencing 16S rDNA and using rep-PCR fingerprinting. In this study, 16S rDNA analysis revealed that most of the isolates were Brenneria. A few belonged to the genus Serratia, suggesting a possible involvement of this bacterium in oak disease. All Spanish isolates of B. quercina were grouped by rep-PCR in a homogenous cluster that differed significantly from the B. quercina reference strains from California. The information obtained by the rep-PCR fingerprint analysis was used to develop PCR-primers for the sensitive and specific detection of B. quercina from infected plant tissues. | |
Internacional
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Si |
JCR del ISI
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Si |
Título de la revista
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PLANT PATHOLOGY |
ISSN
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0032-0862 |
Factor de impacto JCR
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2,012 |
Información de impacto
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Volumen
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57 |
DOI
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Número de revista
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0 |
Desde la página
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812 |
Hasta la página
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819 |
Mes
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ENERO |
Ranking
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