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Memorias de investigación
Ponencias en congresos:
Epigenetic analysis of chrysanthemum shoots regenerated from cryopreserved apices: employment of CRED-RA technique
Año:2009
Áreas de investigación
  • Producción vegetal
Datos
Descripción
The aim of this study has been to apply and to assess the capacity of the CRED-RA technique (¿Coupled restriction enzyme digestion and random amplification¿) (Cai et al., 1996) to obtain the methylation patterns of chrysanthemum cryopreserved shoots, and by comparing methylation patterns to analyse the epigenetic stability of the cryopreserved material in relation to control non-treated tissues. The methodology of this technique is based on the capacity of certain restriction enzymes (Hpa II and Msp I) to recognize the same cut sequence, but with different restriction capacity depending on the methylation status of the cytosine residues. Subsequently, PCR amplifications, using arbitrary primers, are produced with the digested DNA. These amplifications could show different bands patterns according to the DNA methylation status of the original sample. The results obtained in this work showed a high genetic and epigenetic stability of the chrysanthemum samples studied. Nevertheless, two out of six samples, derived from the encapsulation-dehydration treatment, presented different band-patterns compared to their controls when the CRED-RA technique was applied. These differences cannot be correlated to a possible somaclonal variation since the RAPD data did not show any genetic change. No variation was detected in the four samples derived from the vitrification method, although to conclude that this protocol implies a higher stability, compared to encapsulation-dehydration method, further analyses would be necessary.
Internacional
Si
Nombre congreso
COST 871, Working Group 2, Integration of cryopreservation in genebank strategies
Tipo de participación
960
Lugar del congreso
Gatersleben, Alemania
Revisores
Si
ISBN o ISSN
0143-2044
DOI
Fecha inicio congreso
07/09/2009
Fecha fin congreso
11/09/2009
Desde la página
85
Hasta la página
85
Título de las actas
CryoLetters 31(1)
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: INÉS GARCÍA GINÉS
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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