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Descripción
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| We have estimated the rate of 2n-pollen formation and fertility in different hybrid genotypes between T. turgidum and the wild species Aegilops geniculata, Ae. triuncialis, Ae. neglecta, Ae. ventricosa and Ae. cylindrica, and also in hybrids between T. aestivum and Ae. geniculata. Nuclear restitution promotes the formation of dyads at the end of meiosis. Then we first estimated the rate of unreduced meiosis by counting dyads at the tetrad microspore stage using aceto-carmine staining. Bigger pollen size is also an indicator of the production of unreduced gametes since the volume of unreduced pollen grains will be approximately twice the usual volume of a pollen grain. Based on this assumption we estimated 2n-pollen viability in mature anthers stained with Alexander as the rate of giant stained grains. Then, these two estimations were compared with seed set after selfing, as a representative parameter of hybrid fertility. The analyses of dyads at the end of meiosis and mature pollen after Alexander¿s staining resulted useful to predict that a hybrid was fully sterile. However, some hybrids produced no seeds despite some fertility had been expected according to these approaches. In these cases, which mainly affected durum wheat hybrids, we observed a decrease in 2n-pollen production estimates from tetrads to pollen stage. Self-fertility was generally low, but 5-10 seeds per 100 spikelets were formed in some hybrid genotypes. | |
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Internacional
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No |
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Nombre congreso
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International Conference on Polyploidy, Hybridization and Biodiversity |
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Tipo de participación
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960 |
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Lugar del congreso
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Saint Malo, Francia |
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Revisores
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Si |
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ISBN o ISSN
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DOI
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Fecha inicio congreso
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17/05/2009 |
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Fecha fin congreso
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20/05/2009 |
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Título de las actas
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