Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Somaclonal variation after cryopreservation. How to detect it?: AFLPs vs. RAPDs
Año:2009
Áreas de investigación
  • Producción vegetal
Datos
Descripción
We have studied the stability along the encapsulation-dehydration cryopreservation process (pre-culture, encapsulation, dehydration, freeze-treatment and recovery), in order to discriminate variation associated with each step. In this new study, besides RAPDs markers, we have also used a highly discriminative technique, AFLP. Using both techniques, clear differences between control and cryopreserved samples have been detected. Furthermore, AFLP pattern profiles showed somaclonal variation associated to different steps along the process. The percentage of polymorphic bands obtained with AFLP markers was higher than with RAPDs, showing that AFLP technique is more powerful and effective to analyze somaclonal variation. A study of the percentage of variation found in each step of the cryopreservation process using both molecular markers techniques was carried out.
Internacional
Si
Nombre congreso
Annual Meeting of The Society for Low Temperature Biology
Tipo de participación
960
Lugar del congreso
Copenague, Dinamarca
Revisores
Si
ISBN o ISSN
0143-2044
DOI
Fecha inicio congreso
11/09/2008
Fecha fin congreso
12/09/2008
Desde la página
87
Hasta la página
87
Título de las actas
CRYOLETTERS 30(1)
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: M.T. CERVERA
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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