Memorias de investigación
Research Publications in journals:
Leishmania infantum: Antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR
Year:2009

Research Areas
  • Molecular biology

Information
Abstract
Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by Q-PCR was carried out. Low concentrations (2 lM) from both cystatins were unable to inhibit promastigote replication. HvCPI5 was toxic for mammalian cells; 0.1 lM reduced by more than 50% the cell viability. On the contrary, HvCPI6 did not exhibit any toxicity for J774 cells up to 6 lM and inhibited the intracellular amastigote multiplication. Dose¿response analysis showed that 4.8 lM HvCPI6 reduced by >90% the intracellular parasite load and had an approximate IC50 value of 1.5 lM. Comparable results were obtained by direct counting of intracellular amastigotes and Q-PCR. Results point towards the direct inhibition of amastigote multiplication by HvCPI6 and the interest of this recombinant cystatin in the chemotherapy of leishmaniasis.
International
Si
JCR
Si
Title
EXPERIMENTAL PARASITOLOGY
ISBN
0014-4894
Impact factor JCR
1,751
Impact info
Volume
123
10.1016/j.exppara.2009.08.015
Journal number
0
From page
341
To page
346
Month
ENERO
Ranking
Participants

Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología