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Memorias de investigación
Artículos en revistas:
Leishmania infantum: Antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR
Año:2009
Áreas de investigación
  • Biología molecular
Datos
Descripción
Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by Q-PCR was carried out. Low concentrations (2 lM) from both cystatins were unable to inhibit promastigote replication. HvCPI5 was toxic for mammalian cells; 0.1 lM reduced by more than 50% the cell viability. On the contrary, HvCPI6 did not exhibit any toxicity for J774 cells up to 6 lM and inhibited the intracellular amastigote multiplication. Dose¿response analysis showed that 4.8 lM HvCPI6 reduced by >90% the intracellular parasite load and had an approximate IC50 value of 1.5 lM. Comparable results were obtained by direct counting of intracellular amastigotes and Q-PCR. Results point towards the direct inhibition of amastigote multiplication by HvCPI6 and the interest of this recombinant cystatin in the chemotherapy of leishmaniasis.
Internacional
Si
JCR del ISI
Si
Título de la revista
EXPERIMENTAL PARASITOLOGY
ISSN
0014-4894
Factor de impacto JCR
1,751
Información de impacto
Volumen
123
DOI
10.1016/j.exppara.2009.08.015
Número de revista
0
Desde la página
341
Hasta la página
346
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: M. Isabel Diaz Rodriguez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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