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Abstract
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| The objective of this study is to induce the nuclear DNA duplication of anther-derived embryos of cork oak (Quercus suber L.) to obtain doubled-haploid plants. Anther culture of this species produces a low percentage (7.78%) of spontaneous diploids, as assessed by flow cytometry. Therefore, three antimitotic agents, colchicine, oryzalin and amiprophos-methyl were applied in vitro on anther-derived cork-oak haploid embryos from six genotypes at different concentrations and treatment durations. Antimitotic toxicity was determined by embryo survival. Efficiency in inducing chromosome doubling of haploid embryos was evaluated by flow cytometry measurements and differences were observed between treatments. Nuclear DNA duplication and embryo survival of cork-oak haploid embryos was most efficiently induced with oryzalin 0.01 mM for 48 h. Around 50 % diploid embryos were obtained. Amiprophos-methyl 0.01 mM also induced chromosome duplication at an acceptable rate, but lower than oryzalin regardless of the duration of the treatment. Colchicine 1.3 or 8.8 mM was the least efficient, with the induction of necrosis and only a small rate of nuclear DNA duplication. | |
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International
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Si |
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JCR
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Si |
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Title
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J PLANT PHYSIOL |
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ISBN
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0176-1617 |
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Impact factor JCR
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2,239 |
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Impact info
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Volume
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164 |
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Journal number
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12 |
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From page
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1595 |
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To page
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1604 |
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Month
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DICIEMBRE |
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Ranking
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